Thursday, 28 April 2011

Production of Bacitracin (a polypeptide antibiotic)

Units for medium preparation
1. Seed cooker and 2. Fermentor:
    Cook and sterilize medium for the seed/production fermentors. The name "cooker" implies "cook meals" for the organisms, which breaks down large molecules such as starch and proteins into small ones suitable for fermentation by heating and alkaline hydrolysis.
3. Hot water tank
  Containing the hot water to be used for the cooker

 Inoculum preparation: by seed tank or intermediate seed tank (to grow the organisms in a smaller culture vessel to be used a seed for a larger vessel)
4. Mash cooler: To cool down the mash from cooker and utilize the heat to heat up water.

Units for inoculum preparation:
5. Intermediate culture tank
     to prepare inoculum for seed tank,
6. seed tank
     for the large fermentor. (The intermediate tank is seeded with flask culture.)

Downstream processing units-- Isolation of the products: by the centrifugal extractor.

7. Filter press: to remove cell debris by filtration. Removal of insoluble components.
8. Char adsorption:  removal of impurities from the product solution. Purification product

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